7OW8

CryoEM structure of the ABC transporter BmrA E504A mutant in complex with ATP-Mg

7OW8 の概要

• エントリーDOI: 10.2210/pdb7ow8/pdb
• EMDBエントリー: 13095 4749
• 分子名称: Multidrug resistance ABC transporter ATP-binding/permease protein BmrA, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: bmra abc transporter complex with atp-mg multidrug resistance, transport protein
• 由来する生物種: Bacillus subtilis (strain 168)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 132042.62

構造登録者

Gobet, A.,Schoehn, G.,Falson, P.,Chaptal, V. (登録日: 2021-06-17, 公開日: 2022-01-19, 最終更新日: 2025-07-09)

主引用文献

Chaptal, V.,Zampieri, V.,Wiseman, B.,Orelle, C.,Martin, J.,Nguyen, K.A.,Gobet, A.,Di Cesare, M.,Magnard, S.,Javed, W.,Eid, J.,Kilburg, A.,Peuchmaur, M.,Marcoux, J.,Monticelli, L.,Hogbom, M.,Schoehn, G.,Jault, J.M.,Boumendjel, A.,Falson, P.
Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter.
Sci Adv, 8:eabg9215-eabg9215, 2022

PubMed Abstract: Multidrug ABC transporters translocate drugs across membranes by a mechanism for which the molecular features of drug release are so far unknown. Here, we resolved three ATP-Mg-bound outward-facing conformations of the (homodimeric) BmrA by x-ray crystallography and single-particle cryo-electron microscopy (EM) in detergent solution, one of them with rhodamine 6G (R6G), a substrate exported by BmrA when overexpressed in . Two R6G molecules bind to the drug-binding cavity at the level of the outer leaflet, between transmembrane (TM) helices 1-2 of one monomer and TM5'-6' of the other. They induce a rearrangement of TM1-2, highlighting a local flexibility that we confirmed by hydrogen/deuterium exchange and molecular dynamics simulations. In the absence of R6G, simulations show a fast postrelease occlusion of the cavity driven by hydrophobicity, while when present, R6G can move within the cavity, maintaining it open.

PubMed: 35080979
DOI: 10.1126/sciadv.abg9215

実験手法

ELECTRON MICROSCOPY (3.5 Å)