7OUI

Structure of C2S2M2-type Photosystem supercomplex from Arabidopsis thaliana (digitonin-extracted)

7OUI の概要

• エントリーDOI: 10.2210/pdb7oui/pdb
• EMDBエントリー: 13078
• 分子名称: Chlorophyll a-b binding protein, chloroplastic, Photosystem II reaction center protein H, Photosystem II reaction center protein I, ... (42 entities in total)
• 機能のキーワード: oxidoreductase, complex, photosynthesis
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 52
• 化学式量合計: 1321043.25

構造登録者

Graca, A.T.,Hall, M.,Persson, K.,Schroder, W.P. (登録日: 2021-06-11, 公開日: 2021-08-18, 最終更新日: 2025-07-02)

主引用文献

Graca, A.T.,Hall, M.,Persson, K.,Schroder, W.P.
High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction.
Sci Rep, 11:15534-15534, 2021

PubMed Abstract: In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated MnCaO-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.

PubMed: 34330992
DOI: 10.1038/s41598-021-94914-x

実験手法

ELECTRON MICROSCOPY (2.79 Å)