7OTD

Oxytocin NMR solution structure

7OTD の概要

• エントリーDOI: 10.2210/pdb7otd/pdb
• 関連するPDBエントリー: 7OFG
• NMR情報: BMRB: 34637
• 分子名称: UNK-TYR-ILE-GLN-ASN-CYS-PRO-LEU-GLY, COPPER (II) ION, AMINO GROUP (3 entities in total)
• 機能のキーワード: natural peptide, cyclic, disulfide bond, copper-binding, hormone
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1089.76

構造登録者

Shalev, D.E.,Alshanski, I.,Yitzchaik, S.,Hurevich, M. (登録日: 2021-06-10, 公開日: 2021-10-13)

主引用文献

Alshanski, I.,Shalev, D.E.,Yitzchaik, S.,Hurevich, M.
Determining the structure and binding mechanism of oxytocin-Cu 2+ complex using paramagnetic relaxation enhancement NMR analysis.
J.Biol.Inorg.Chem., 26:809-815, 2021

PubMed Abstract: Oxytocin is a neuropeptide that binds copper ions in nature. The structure of oxytocin in interaction with Cu was determined here by NMR, showing which atoms of the peptide are involved in binding. Paramagnetic relaxation enhancement NMR analyses indicated a binding mechanism where the amino terminus was required for binding and subsequently Tyr2, Ile3 and Gln4 bound in that order. The aromatic ring of Tyr2 formed a π-cation interaction with Cu. Oxytocin copper complex structure revealed by paramagnetic relaxation enhancement NMR analyses.

PubMed: 34459989
DOI: 10.1007/s00775-021-01897-1

実験手法

SOLUTION NMR