7OSK

Ignisphaera aggregans GH53 catalytic domain

Summary for 7OSK

• Entry DOI: 10.2210/pdb7osk/pdb
• Descriptor: Arabinogalactan endo-1,4-beta-galactosidase, CALCIUM ION, HEXANE-1,6-DIOL, ... (5 entities in total)
• Functional Keywords: galactanase, thermostable, hydrolase
• Biological source: Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
• Total number of polymer chains: 2
• Total formula weight: 92958.66

Authors

Fredslund, F.,Lo Leggio, L.,Poulsen, J.C.,Rasmussen, K.K.,Muderspach, S.,Krogh, K.B.R.M.,Jensen, K. (deposition date: 2021-06-08, release date: 2021-09-29, Last modification date: 2024-01-31)

Primary citation

Muderspach, S.J.,Fredslund, F.,Volf, V.,Poulsen, J.N.,Blicher, T.H.,Clausen, M.H.,Rasmussen, K.K.,Krogh, K.B.R.M.,Jensen, K.,Lo Leggio, L.
Engineering the substrate binding site of the hyperthermostable archaeal endo-beta-1,4-galactanase from Ignisphaera aggregans.
Biotechnol Biofuels, 14:183-183, 2021

PubMed Abstract: Endo-β-1,4-galactanases are glycoside hydrolases (GH) from the GH53 family belonging to the largest clan of GHs, clan GH-A. GHs are ubiquitous and involved in a myriad of biological functions as well as being widely used industrially. Endo-β-1,4-galactanases, in particular hydrolyse galactan and arabinogalactan in pectin, a major component of the primary plant cell wall, with important functions in plant defence and application in the food and other industries. Here, we explore the family's biological diversity by characterizing the first archaeal and hyperthermophilic GH53 galactanase, and utilize it as a scaffold for engineering enzymes with different product lengths.

PubMed: 34530892
DOI: 10.1186/s13068-021-02025-6

Experimental method

X-RAY DIFFRACTION (2.65 Å)