7OQT

G-quadruplex structure of the C. elegans telomeric repeat: A two tetrads basket type conformation stabilised by a Hoogsteen C-T base-pair

Summary for 7OQT

• Entry DOI: 10.2210/pdb7oqt/pdb
• NMR Information: BMRB: 34631
• Descriptor: DNA (5'-D(*GP*GP*CP*TP*TP*AP*GP*GP*CP*TP*TP*AP*GP*GP*CP*TP*TP*AP*GP*G)-3'), POTASSIUM ION (2 entities in total)
• Functional Keywords: g-quadruplex, c. elegans telomere, dna
• Biological source: Caenorhabditis elegans
• Total number of polymer chains: 1
• Total formula weight: 6260.11

Authors

Marquevielle, J.,De Rache, A.,Amrane, S. (deposition date: 2021-06-04, release date: 2022-06-22, Last modification date: 2024-06-19)

Primary citation

Marquevielle, J.,De Rache, A.,Vialet, B.,Morvan, E.,Mergny, J.L.,Amrane, S.
G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C-T base-pair.
Nucleic Acids Res., 50:7134-7146, 2022

PubMed Abstract: The Caenorhabditis elegans model has greatly contributed to the understanding of the role of G-quadruplexes in genomic instability. The GGCTTA repeats of the C. elegans telomeres resemble the GGGTTA repeats of the human telomeres. However, the comparison of telomeric sequences (Homo sapiens, Tetrahymena, Oxytricha, Bombyx mori and Giardia) revealed that small changes in these repeats can drastically change the topology of the folded G-quadruplex. In the present work we determined the structure adopted by the C. elegans telomeric sequence d[GG(CTTAGG)3]. The investigated C. elegans telomeric sequence is shown to fold into an intramolecular two G-tetrads basket type G-quadruplex structure that includes a C-T base pair in the diagonal loop. This work sheds light on the telomeric structure of the widely used C. elegans animal model.

PubMed: 35736226
DOI: 10.1093/nar/gkac523

Experimental method

SOLUTION NMR