7OQD

A single sulfatase is required for metabolism of colonic mucin O-glycans and intestinal colonization by a symbiotic human gut bacterium (BT1636-S1_20)

これはPDB形式変換不可エントリーです。

7OQD の概要

• エントリーDOI: 10.2210/pdb7oqd/pdb
• 分子名称: Arylsulfatase, 3-O-sulfo-beta-D-galactopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: carbohydrate sulfatase, mucin, microbiome, bacteroides, hydrolase
• 由来する生物種: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58329.61

構造登録者

Sofia de Jesus Vaz Luis, A.,Basle, A.,Martens, E.C.,Cartmell, A. (登録日: 2021-06-03, 公開日: 2021-10-27, 最終更新日: 2024-01-31)

主引用文献

Luis, A.S.,Jin, C.,Pereira, G.V.,Glowacki, R.W.P.,Gugel, S.R.,Singh, S.,Byrne, D.P.,Pudlo, N.A.,London, J.A.,Basle, A.,Reihill, M.,Oscarson, S.,Eyers, P.A.,Czjzek, M.,Michel, G.,Barbeyron, T.,Yates, E.A.,Hansson, G.C.,Karlsson, N.G.,Cartmell, A.,Martens, E.C.
A single sulfatase is required to access colonic mucin by a gut bacterium.
Nature, 598:332-337, 2021

PubMed Abstract: Humans have co-evolved with a dense community of microbial symbionts that inhabit the lower intestine. In the colon, secreted mucus creates a barrier that separates these microorganisms from the intestinal epithelium. Some gut bacteria are able to utilize mucin glycoproteins, the main mucus component, as a nutrient source. However, it remains unclear which bacterial enzymes initiate degradation of the complex O-glycans found in mucins. In the distal colon, these glycans are heavily sulfated, but specific sulfatases that are active on colonic mucins have not been identified. Here we show that sulfatases are essential to the utilization of distal colonic mucin O-glycans by the human gut symbiont Bacteroides thetaiotaomicron. We characterized the activity of 12 different sulfatases produced by this species, showing that they are collectively active on all known sulfate linkages in O-glycans. Crystal structures of three enzymes provide mechanistic insight into the molecular basis of substrate specificity. Unexpectedly, we found that a single sulfatase is essential for utilization of sulfated O-glycans in vitro and also has a major role in vivo. Our results provide insight into the mechanisms of mucin degradation by a prominent group of gut bacteria, an important process for both normal microbial gut colonization and diseases such as inflammatory bowel disease.

PubMed: 34616040
DOI: 10.1038/s41586-021-03967-5

実験手法

X-RAY DIFFRACTION (2.3 Å)