7OO8

Mechanosensitive channel MscS solubilized with LMNG in closed conformation with added lipid

7OO8 の概要

• エントリーDOI: 10.2210/pdb7oo8/pdb
• EMDBエントリー: 12996 12997 13003 13006 13007
• 分子名称: Mechanosensitive channel of small conductance (MscS) (1 entity in total)
• 機能のキーワード: mechanosensitive channel, lmng, delipidation, membrane protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 223958.27

構造登録者

Rasmussen, T.,Flegler, V.J.,Boettcher, B. (登録日: 2021-05-26, 公開日: 2021-09-01, 最終更新日: 2024-07-10)

主引用文献

Flegler, V.J.,Rasmussen, A.,Borbil, K.,Boten, L.,Chen, H.A.,Deinlein, H.,Halang, J.,Hellmanzik, K.,Loffler, J.,Schmidt, V.,Makbul, C.,Kraft, C.,Hedrich, R.,Rasmussen, T.,Bottcher, B.
Mechanosensitive channel gating by delipidation.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: The mechanosensitive channel of small conductance (MscS) protects bacteria against hypoosmotic shock. It can sense the tension in the surrounding membrane and releases solutes if the pressure in the cell is getting too high. The membrane contacts MscS at sensor paddles, but lipids also leave the membrane and move along grooves between the paddles to reside as far as 15 Å away from the membrane in hydrophobic pockets. One sensing model suggests that a higher tension pulls lipids from the grooves back to the membrane, which triggers gating. However, it is still unclear to what degree this model accounts for sensing and what contribution the direct interaction of the membrane with the channel has. Here, we show that MscS opens when it is sufficiently delipidated by incubation with the detergent dodecyl-β-maltoside or the branched detergent lauryl maltose neopentyl glycol. After addition of detergent-solubilized lipids, it closes again. These results support the model that lipid extrusion causes gating: Lipids are slowly removed from the grooves and pockets by the incubation with detergent, which triggers opening. Addition of lipids in micelles allows lipids to migrate back into the pockets, which closes the channel even in the absence of a membrane. Based on the distribution of the aliphatic chains in the open and closed conformation, we propose that during gating, lipids leave the complex on the cytosolic leaflet at the height of highest lateral tension, while on the periplasmic side, lipids flow into gaps, which open between transmembrane helices.

PubMed: 34376558
DOI: 10.1073/pnas.2107095118

実験手法

ELECTRON MICROSCOPY (3.7 Å)