7OKT

X-ray structure of soluble EPCR in C2221 space group

7OKT の概要

• エントリーDOI: 10.2210/pdb7okt/pdb
• 分子名称: Endothelial protein C receptor, alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: mhc class i-like, phospholipid, anticoagulant, endothelial cell membrane receptor, lipid binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47405.00

構造登録者

Erausquin, E.,Dichiara, M.G.,Lopez-Sagaseta, J. (登録日: 2021-05-18, 公開日: 2022-04-27, 最終更新日: 2024-10-09)

主引用文献

Erausquin, E.,Moran-Garrido, M.,Saiz, J.,Barbas, C.,Dichiara-Rodriguez, G.,Urdiciain, A.,Lopez-Sagaseta, J.
Identification of a broad lipid repertoire associated to the endothelial cell protein C receptor (EPCR).
Sci Rep, 12:15127-15127, 2022

PubMed Abstract: Evidence is mounting that the nature of the lipid bound to the endothelial cell protein C receptor (EPCR) has an impact on its biological roles, as observed in anticoagulation and more recently, in autoimmune disease. Phosphatidylethanolamine and phosphatidylcholine species dominate the EPCR lipid cargo, yet, the extent of diversity in the EPCR-associated lipid repertoire is still unknown and remains to be uncovered. We undertook mass spectrometry analyses to decipher the EPCR lipidome, and identified species not yet described as EPCR ligands, such as phosphatidylinositols and phosphatidylserines. Remarkably, we found further, more structurally divergent lipids classes, represented by ceramides and sphingomyelins, both in less abundant quantities. In support of our mass spectrometry results and previous studies, high-resolution crystal structures of EPCR in three different space groups point to a prevalent diacyl phospholipid moiety in EPCR's pocket but a mobile and ambiguous lipid polar head group. In sum, these studies indicate that EPCR can associate with varied lipid classes, which might impact its properties in anticoagulation and the onset of autoimmune disease.

PubMed: 36068249
DOI: 10.1038/s41598-022-18844-y

実験手法

X-RAY DIFFRACTION (1.95 Å)