7OJ7

Crystal structure of human coxsackievirus A24v in complex with a pentavalent N-acetylneuraminic acid conjugate

これはPDB形式変換不可エントリーです。

7OJ7 の概要

• エントリーDOI: 10.2210/pdb7oj7/pdb
• 分子名称: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (8 entities in total)
• 機能のキーワード: human coxsackievirus a24v, starfish-like inhibitor, corannulene, virus
• 由来する生物種: Coxsackievirus A24; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98886.35

構造登録者

Zocher, G.,Stehle, T. (登録日: 2021-05-14, 公開日: 2021-10-13, 最終更新日: 2024-01-31)

主引用文献

Johansson, E.,Caraballo, R.,Hurdiss, D.L.,Mistry, N.,Andersson, C.D.,Thompson, R.F.,Ranson, N.A.,Zocher, G.,Stehle, T.,Arnberg, N.,Elofsson, M.
Exploring the Effect of Structure-Based Scaffold Hopping on the Inhibition of Coxsackievirus A24v Transduction by Pentavalent N-Acetylneuraminic Acid Conjugates.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Coxsackievirus A24 variant (CVA24v) is the primary causative agent of the highly contagious eye infection designated acute hemorrhagic conjunctivitis (AHC). It is solely responsible for two pandemics and several recurring outbreaks of the disease over the last decades, thus affecting millions of individuals throughout the world. To date, no antiviral agents or vaccines are available for combating this disease, and treatment is mainly supportive. CVA24v utilizes Neu5Ac-containing glycans as attachment receptors facilitating entry into host cells. We have previously reported that pentavalent Neu5Ac conjugates based on a glucose-scaffold inhibit CVA24v infection of human corneal epithelial cells. In this study, we report on the design and synthesis of scaffold-replaced pentavalent Neu5Ac conjugates and their effect on CVA24v cell transduction and the use of cryogenic electron microscopy (cryo-EM) to study the binding of these multivalent conjugates to CVA24v. The results presented here provide insights into the development of Neu5Ac-based inhibitors of CVA24v and, most significantly, the first application of cryo-EM to study the binding of a multivalent ligand to a lectin.

PubMed: 34445134
DOI: 10.3390/ijms22168418

実験手法

X-RAY DIFFRACTION (1.78 Å)