7OHD

CRYSTAL STRUCTURE OF FERRIC MURINE NEUROGLOBIN CDLESS MUTANT

7OHD の概要

• エントリーDOI: 10.2210/pdb7ohd/pdb
• 分子名称: Neuroglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: heme protein, oxygen transport
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33340.56

構造登録者

Exertier, C.,Freda, I.,Montemiglio, L.C.,Savino, C.,Cerutti, G.,Gugole, E.,Vallone, B. (登録日: 2021-05-10, 公開日: 2022-07-13, 最終更新日: 2024-01-31)

主引用文献

Exertier, C.,Sebastiani, F.,Freda, I.,Gugole, E.,Cerutti, G.,Parisi, G.,Montemiglio, L.C.,Becucci, M.,Viappiani, C.,Bruno, S.,Savino, C.,Zamparelli, C.,Anselmi, M.,Abbruzzetti, S.,Smulevich, G.,Vallone, B.
Probing the Role of Murine Neuroglobin CDloop-D-Helix Unit in CO Ligand Binding and Structural Dynamics.
Acs Chem.Biol., 17:2099-2108, 2022

PubMed Abstract: We produced a neuroglobin variant, namely, Ngb CDless, with the excised CDloop- and D-helix, directly joining the C- and E-helices. The CDless variant retained bis-His hexacoordination, and we investigated the role of the CDloop-D-helix unit in controlling the CO binding and structural dynamics by an integrative approach based on X-ray crystallography, rapid mixing, laser flash photolysis, resonance Raman spectroscopy, and molecular dynamics simulations. Rapid mixing and laser flash photolysis showed that ligand affinity was unchanged with respect to the wild-type protein, albeit with increased on and off constants for rate-limiting heme iron hexacoordination by the distal His64. Accordingly, resonance Raman spectroscopy highlighted a more open distal pocket in the CO complex that, in agreement with MD simulations, likely involves His64 swinging inward and outward of the distal heme pocket. Ngb CDless displays a more rigid overall structure with respect to the wild type, abolishing the structural dynamics of the CDloop-D-helix hypothesized to mediate its signaling role, and it retains ligand binding control by distal His64. In conclusion, this mutant may represent a tool to investigate the involvement of CDloop-D-helix in neuroprotective signaling in a cellular or animal model.

PubMed: 35797699
DOI: 10.1021/acschembio.2c00172

実験手法

X-RAY DIFFRACTION (1.8 Å)