7OGR

Structure of the apo-state of the bacteriophage PhiKZ non-virion RNA polymerase

7OGR の概要

• エントリーDOI: 10.2210/pdb7ogr/pdb
• EMDBエントリー: 12885 12886
• 分子名称: UNK helices, PHIKZ055, PHIKZ068, ... (7 entities in total)
• 機能のキーワード: rna polymerase, phikz, non-virion, transcription
• 由来する生物種: Pseudomonas phage phiKZ; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 339456.16

構造登録者

de Martin Garrido, N.,Lai Wan Loong, Y.T.E.,Yakunina, M.,Aylett, C.H.S. (登録日: 2021-05-07, 公開日: 2021-07-07, 最終更新日: 2023-11-22)

主引用文献

deYMartin Garrido, N.,Orekhova, M.,Lai Wan Loong, Y.T.E.,Litvinova, A.,Ramlaul, K.,Artamonova, T.,Melnikov, A.S.,Serdobintsev, P.,Aylett, C.H.S.,Yakunina, M.
Structure of the bacteriophage PhiKZ non-virion RNA polymerase.
Nucleic Acids Res., 49:7732-7739, 2021

PubMed Abstract: Bacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. ΦKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a 'nucleus', erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. ΦKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. ΦKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial β/β' subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of ΦKZ nvRNAP to better than 3.0 Å, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to σ-factor.

PubMed: 34181731
DOI: 10.1093/nar/gkab539

実験手法

ELECTRON MICROSCOPY (3 Å)