7OG6

Structure of Alternanthera Mosaic VLP by cryoEM

7OG6 の概要

• エントリーDOI: 10.2210/pdb7og6/pdb
• EMDBエントリー: 12879
• 分子名称: Coat protein, RNA (5'-R(P*UP*UP*UP*UP*U)-3') (2 entities in total)
• 機能のキーワード: potexvirus, virus like particle, vlp.
• 由来する生物種: Alternanthera mosaic virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23729.77

構造登録者

Byrne, M.J.,Ranson, N.A.,Lomonossoff, G.P.,Thuenemann, E.C. (登録日: 2021-05-06, 公開日: 2021-06-23, 最終更新日: 2025-07-09)

主引用文献

Thuenemann, E.C.,Byrne, M.J.,Peyret, H.,Saunders, K.,Castells-Graells, R.,Ferriol, I.,Santoni, M.,Steele, J.F.C.,Ranson, N.A.,Avesani, L.,Lopez-Moya, J.J.,Lomonossoff, G.P.
A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.
Viruses, 13:-, 2021

PubMed Abstract: The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.

PubMed: 34064959
DOI: 10.3390/v13050885

実験手法

ELECTRON MICROSCOPY (3.3 Å)