7OG4

Human mitochondrial ribosome in complex with P/E-tRNA

これはPDB形式変換不可エントリーです。

「6ZSF」から置き換えられました

7OG4 の概要

• エントリーDOI: 10.2210/pdb7og4/pdb
• EMDBエントリー: 12877
• 分子名称: 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L41, mitochondrial, 16S mitochondrial rRNA, ... (90 entities in total)
• 機能のキーワード: mitochondria, ribosome, trna, mrna, translation
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 90
• 化学式量合計: 3041262.25

構造登録者

Aibara, S.,Singh, V.,Modelska, A.,Amunts, A. (登録日: 2021-05-06, 公開日: 2021-06-23, 最終更新日: 2024-11-13)

主引用文献

Aibara, S.,Singh, V.,Modelska, A.,Amunts, A.
Structural basis of mitochondrial translation.
Elife, 9:-, 2020

PubMed Abstract: Translation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the binding of mt-mRNA and to what extent they are involved in the translocation of transfer RNA (mt-tRNA) is unclear. To visualize the process of translation in human mitochondria, we report ~3.0 Å resolution structure of the human mitoribosome, including the L7/L12 stalk, and eight structures of its functional complexes with mt-mRNA, mt-tRNAs, recycling factor and additional trans factors. The study reveals a transacting protein module LRPPRC-SLIRP that delivers mt-mRNA to the mitoribosomal small subunit through a dedicated platform formed by the mitochondria-specific protein mS39. Mitoribosomal proteins of the large subunit mL40, mL48, and mL64 coordinate translocation of mt-tRNA. The comparison between those structures shows dynamic interactions between the mitoribosome and its ligands, suggesting a sequential mechanism of conformational changes.

PubMed: 32812867
DOI: 10.7554/eLife.58362

実験手法

ELECTRON MICROSCOPY (3.8 Å)