7OE0

E. coli pre-30S delta rbfA ribosomal subunit class F

7OE0 の概要

• エントリーDOI: 10.2210/pdb7oe0/pdb
• EMDBエントリー: 12856
• 分子名称: 30S ribosomal protein S2, 30S ribosomal protein S17, 30S ribosomal protein S18, ... (21 entities in total)
• 機能のキーワード: ribosome, 30s subunit, rna, rbfa
• 由来する生物種: Escherichia coli BW25113; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 779045.16

構造登録者

Maksimova, E.,Korepanov, A.,Baymukhametov, T.,Kravchenko, O.,Stolboushkina, E. (登録日: 2021-04-30, 公開日: 2021-07-14, 最終更新日: 2024-07-10)

主引用文献

Maksimova, E.M.,Korepanov, A.P.,Kravchenko, O.V.,Baymukhametov, T.N.,Myasnikov, A.G.,Vassilenko, K.S.,Afonina, Z.A.,Stolboushkina, E.A.
RbfA Is Involved in Two Important Stages of 30S Subunit Assembly: Formation of the Central Pseudoknot and Docking of Helix 44 to the Decoding Center.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Ribosome biogenesis is a highly coordinated and complex process that requires numerous assembly factors that ensure prompt and flawless maturation of ribosomal subunits. Despite the increasing amount of data collected, the exact role of most assembly factors and mechanistic details of their operation remain unclear, mainly due to the shortage of high-resolution structural information. Here, using cryo-electron microscopy, we characterized 30S ribosomal particles isolated from an   strain with a deleted gene for the RbfA factor. The cryo-EM maps for pre-30S subunits were divided into six classes corresponding to consecutive assembly intermediates: from the particles with a completely unresolved head domain and unfolded central pseudoknot to almost mature 30S subunits with well-resolved body, platform, and head domains and partially distorted helix 44. The structures of two predominant 30S intermediates belonging to most populated classes obtained at 2.7 Å resolutions indicate that RbfA acts at two distinctive 30S assembly stages: early formation of the central pseudoknot including folding of the head, and positioning of helix 44 in the decoding center at a later stage. Additionally, it was shown that the formation of the central pseudoknot may promote stabilization of the head domain, likely through the RbfA-dependent maturation of the neck helix 28. An update to the model of factor-dependent 30S maturation is proposed, suggesting that RfbA is involved in most of the subunit assembly process.

PubMed: 34200244
DOI: 10.3390/ijms22116140

実験手法

ELECTRON MICROSCOPY (2.69 Å)