7OCS
Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD-D16A from Acinetobacter baumannii
7OCS の概要
エントリーDOI | 10.2210/pdb7ocs/pdb |
分子名称 | HAD hydrolase, family IA, variant 3, D-Mannitol-1-phosphate, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (14 entities in total) |
機能のキーワード | mannitol, dehydrogenase, phosphatase, nadph, fructose-6-phosphate, oxidoreductase, had hydrolase family ia variant 3 |
由来する生物種 | Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 338584.54 |
構造登録者 | |
主引用文献 | Tam, H.K.,Konig, P.,Himpich, S.,Ngu, N.D.,Abele, R.,Muller, V.,Pos, K.M. Unidirectional mannitol synthesis of Acinetobacter baumannii MtlD is facilitated by the helix-loop-helix-mediated dimer formation. Proc.Natl.Acad.Sci.USA, 119:e2107994119-e2107994119, 2022 Cited by PubMed Abstract: Persistence of Acinetobacter baumannii in environments with low water activity is largely attributed to the biosynthesis of compatible solutes. Mannitol is one of the key compatible solutes in A. baumannii, and it is synthesized by a bifunctional mannitol-1-phosphate dehydrogenase/phosphatase (AbMtlD). AbMtlD catalyzes the conversion of fructose-6-phosphate to mannitol in two consecutive steps. Here, we report the crystal structure of dimeric AbMtlD, constituting two protomers each with a dehydrogenase and phosphatase domain. A proper assembly of AbMtlD dimer is facilitated by an intersection comprising a unique helix–loop–helix (HLH) domain. Reduction and dephosphorylation catalysis of fructose-6-phosphate to mannitol is dependent on the transient dimerization of AbMtlD. AbMtlD presents as a monomer under lower ionic strength conditions and was found to be mainly dimeric under high-salt conditions. The AbMtlD catalytic efficiency was markedly increased by cross-linking the protomers at the intersected HLH domain via engineered disulfide bonds. Inactivation of the AbMtlD phosphatase domain results in an intracellular accumulation of mannitol-1-phosphate in A. baumannii, leading to bacterial growth impairment upon salt stress. Taken together, our findings demonstrate that salt-induced dimerization of the bifunctional AbMtlD increases catalytic dehydrogenase and phosphatase efficiency, resulting in unidirectional catalysis of mannitol production. PubMed: 35363566DOI: 10.1073/pnas.2107994119 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.3 Å) |
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