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7OCS

Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD-D16A from Acinetobacter baumannii

7OCS の概要
エントリーDOI10.2210/pdb7ocs/pdb
分子名称HAD hydrolase, family IA, variant 3, D-Mannitol-1-phosphate, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (14 entities in total)
機能のキーワードmannitol, dehydrogenase, phosphatase, nadph, fructose-6-phosphate, oxidoreductase, had hydrolase family ia variant 3
由来する生物種Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841
タンパク質・核酸の鎖数4
化学式量合計338584.54
構造登録者
Tam, H.K.,Mueller, V.,Pos, K.M. (登録日: 2021-04-28, 公開日: 2022-04-27, 最終更新日: 2024-01-31)
主引用文献Tam, H.K.,Konig, P.,Himpich, S.,Ngu, N.D.,Abele, R.,Muller, V.,Pos, K.M.
Unidirectional mannitol synthesis of Acinetobacter baumannii MtlD is facilitated by the helix-loop-helix-mediated dimer formation.
Proc.Natl.Acad.Sci.USA, 119:e2107994119-e2107994119, 2022
Cited by
PubMed Abstract: Persistence of Acinetobacter baumannii in environments with low water activity is largely attributed to the biosynthesis of compatible solutes. Mannitol is one of the key compatible solutes in A. baumannii, and it is synthesized by a bifunctional mannitol-1-phosphate dehydrogenase/phosphatase (AbMtlD). AbMtlD catalyzes the conversion of fructose-6-phosphate to mannitol in two consecutive steps. Here, we report the crystal structure of dimeric AbMtlD, constituting two protomers each with a dehydrogenase and phosphatase domain. A proper assembly of AbMtlD dimer is facilitated by an intersection comprising a unique helix–loop–helix (HLH) domain. Reduction and dephosphorylation catalysis of fructose-6-phosphate to mannitol is dependent on the transient dimerization of AbMtlD. AbMtlD presents as a monomer under lower ionic strength conditions and was found to be mainly dimeric under high-salt conditions. The AbMtlD catalytic efficiency was markedly increased by cross-linking the protomers at the intersected HLH domain via engineered disulfide bonds. Inactivation of the AbMtlD phosphatase domain results in an intracellular accumulation of mannitol-1-phosphate in A. baumannii, leading to bacterial growth impairment upon salt stress. Taken together, our findings demonstrate that salt-induced dimerization of the bifunctional AbMtlD increases catalytic dehydrogenase and phosphatase efficiency, resulting in unidirectional catalysis of mannitol production.
PubMed: 35363566
DOI: 10.1073/pnas.2107994119
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 7ocs
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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