7O8H

NmHR light state structure at 10 ns after photoexcitation determined by serial femtosecond crystallography (with extrapolated, dark and light dataset)

7O8H の概要

• エントリーDOI: 10.2210/pdb7o8h/pdb
• 分子名称: Chloride pumping rhodopsin, RETINAL, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: chloride pump, rhodopsin, membrane protein
• 由来する生物種: Nonlabens marinus S1-08
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35432.79

構造登録者

Mous, S.,Gotthard, G.,Ehrenberg, D.,Sen, S.,James, D.,Johnson, P.,Weinert, T.,Nass, K.,Furrer, A.,Kekilli, D.,Ma, P.,Bruenle, S.,Casadei, C.,Martiel, I.,Dworkowski, F.,Gashi, D.,Skopintsev, P.,Wranik, M.,Knopp, G.,Panepucci, E.,Panneels, V.,Cirelli, C.,Ozerov, D.,Schertler, G.,Wang, M.,Milne, C.,Standfuss, J.,Schapiro, I.,Heberle, J.,Nogly, P. (登録日: 2021-04-15, 公開日: 2022-02-09, 最終更新日: 2024-11-20)

主引用文献

Mous, S.,Gotthard, G.,Ehrenberg, D.,Sen, S.,Weinert, T.,Johnson, P.J.M.,James, D.,Nass, K.,Furrer, A.,Kekilli, D.,Ma, P.,Brunle, S.,Casadei, C.M.,Martiel, I.,Dworkowski, F.,Gashi, D.,Skopintsev, P.,Wranik, M.,Knopp, G.,Panepucci, E.,Panneels, V.,Cirelli, C.,Ozerov, D.,Schertler, G.F.X.,Wang, M.,Milne, C.,Standfuss, J.,Schapiro, I.,Heberle, J.,Nogly, P.
Dynamics and mechanism of a light-driven chloride pump.
Science, 375:845-851, 2022

PubMed Abstract: Chloride transport by microbial rhodopsins is an essential process for which molecular details such as the mechanisms that convert light energy to drive ion pumping and ensure the unidirectionality of the transport have remained elusive. We combined time-resolved serial crystallography with time-resolved spectroscopy and multiscale simulations to elucidate the molecular mechanism of a chloride-pumping rhodopsin and the structural dynamics throughout the transport cycle. We traced transient anion-binding sites, obtained evidence for how light energy is used in the pumping mechanism, and identified steric and electrostatic molecular gates ensuring unidirectional transport. An interaction with the π-electron system of the retinal supports transient chloride ion binding across a major bottleneck in the transport pathway. These results allow us to propose key mechanistic features enabling finely controlled chloride transport across the cell membrane in this light-powered chloride ion pump.

PubMed: 35113649
DOI: 10.1126/science.abj6663

実験手法

X-RAY DIFFRACTION (1.8 Å)