7O6P

Structure of the borneol dehydrogenase 2 of Salvia officinalis

7O6P の概要

• エントリーDOI: 10.2210/pdb7o6p/pdb
• EMDBエントリー: 12739
• 分子名称: borneol dehydrogenase (2 entities in total)
• 機能のキーワード: terpenoid, alcohol, oxidoreductase, borneol, rossmann-like fold
• 由来する生物種: Salvia officinalis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129070.41

構造登録者

Dimos, N.,Helmer, C.P.O.,Hilal, T.,Loll, B. (登録日: 2021-04-12, 公開日: 2021-12-01, 最終更新日: 2025-07-02)

主引用文献

Dimos, N.,Helmer, C.P.O.,Chanique, A.M.,Wahl, M.C.,Kourist, R.,Hilal, T.,Loll, B.
CryoEM analysis of small plant biocatalysts at sub-2 angstrom resolution.
Acta Crystallogr D Struct Biol, 78:113-123, 2022

PubMed Abstract: Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Å resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.

PubMed: 34981767
DOI: 10.1107/S205979832101216X

実験手法

ELECTRON MICROSCOPY (2.04 Å)