7O3V

Stalk complex structure (TrwJ/VirB5-TrwI/VirB6) from the fully-assembled R388 type IV secretion system determined by cryo-EM.

7O3V の概要

• エントリーDOI: 10.2210/pdb7o3v/pdb
• EMDBエントリー: 12709
• 分子名称: TrwJ protein, TrwI protein (2 entities in total)
• 機能のキーワード: type iv secretion system, type 4 secretion system, t4ss, stalk, stalk complex, inner membrane, r388 plasmid, conjugation, bacterial secretion, secretion, secretion system, protein complex, virb5, virb6, trwj, trwi, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 302573.16

構造登録者

Mace, K.,Vadakkepat, A.K.,Lukoyanova, N.,Waksman, G. (登録日: 2021-04-03, 公開日: 2022-06-22, 最終更新日: 2024-07-10)

主引用文献

Mace, K.,Vadakkepat, A.K.,Redzej, A.,Lukoyanova, N.,Oomen, C.,Braun, N.,Ukleja, M.,Lu, F.,Costa, T.R.D.,Orlova, E.V.,Baker, D.,Cong, Q.,Waksman, G.
Cryo-EM structure of a type IV secretion system.
Nature, 607:191-196, 2022

PubMed Abstract: Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell. It is the primary means by which antibiotic resistance genes spread among bacterial populations. In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer. Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili.

PubMed: 35732732
DOI: 10.1038/s41586-022-04859-y

実験手法

ELECTRON MICROSCOPY (3.7 Å)