7O3D

Cooperation between the intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly

7O3D の概要

• エントリーDOI: 10.2210/pdb7o3d/pdb
• EMDBエントリー: 12704
• 分子名称: Transcription elongation factor SPT6 (1 entity in total)
• 機能のキーワード: spt6, elongation factor, rna pol ii, nucleosome, ctd, chaperone
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 134923.03

構造登録者

Kasiliauskaite, A.,Kubicek, K.,Klumpler, T.,Zanova, M.,Zapletal, D.,Novacek, J.,Stefl, R. (登録日: 2021-04-01, 公開日: 2022-04-13, 最終更新日: 2024-11-13)

主引用文献

Kasiliauskaite, A.,Kubicek, K.,Klumpler, T.,Zanova, M.,Zapletal, D.,Koutna, E.,Novacek, J.,Stefl, R.
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.
Nucleic Acids Res., 50:5961-5973, 2022

PubMed Abstract: Transcription elongation factor Spt6 associates with RNA polymerase II (Pol II) and acts as a histone chaperone, which promotes the reassembly of nucleosomes following the passage of Pol II. The precise mechanism of nucleosome reassembly mediated by Spt6 remains unclear. In this study, we used a hybrid approach combining cryo-electron microscopy and small-angle X-ray scattering to visualize the architecture of Spt6 from Saccharomyces cerevisiae. The reconstructed overall architecture of Spt6 reveals not only the core of Spt6, but also its flexible N- and C-termini, which are critical for Spt6's function. We found that the acidic N-terminal region of Spt6 prevents the binding of Spt6 not only to the Pol II CTD and Pol II CTD-linker, but also to pre-formed intact nucleosomes and nucleosomal DNA. The N-terminal region of Spt6 self-associates with the tSH2 domain and the core of Spt6 and thus controls binding to Pol II and nucleosomes. Furthermore, we found that Spt6 promotes the assembly of nucleosomes in vitro. These data indicate that the cooperation between the intrinsically disordered and structured regions of Spt6 regulates nucleosome and Pol II CTD binding, and also nucleosome assembly.

PubMed: 35640611
DOI: 10.1093/nar/gkac451

実験手法

ELECTRON MICROSCOPY (3.71 Å)