7O3C

Murine supercomplex CIII2CIV in the mature unlocked conformation

7O3C の概要

• エントリーDOI: 10.2210/pdb7o3c/pdb
• 関連するPDBエントリー: 7O37
• EMDBエントリー: 12703
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 10, Cytochrome b-c1 complex subunit 9, ... (37 entities in total)
• 機能のキーワード: mitochondria, respiratory chain, supercomplex, oxidoreductase, complex iii, complex iv, membrane protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 716988.62

構造登録者

Vercellino, I.,Sazanov, L.A. (登録日: 2021-04-01, 公開日: 2021-10-13, 最終更新日: 2024-10-23)

主引用文献

Vercellino, I.,Sazanov, L.A.
Structure and assembly of the mammalian mitochondrial supercomplex CIII 2 CIV.
Nature, 598:364-367, 2021

PubMed Abstract: The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes, whose precise role is debated. Supercomplexes CIIICIV (refs. ), CICIII (ref. ) and CICIIICIV (respirasome) exist in mammals, but in contrast to CICIII and the respirasome, to date the only known eukaryotic structures of CIIICIV come from Saccharomyces cerevisiae and plants, which have different organization. Here we present the first, to our knowledge, structures of mammalian (mouse and ovine) CIIICIV and its assembly intermediates, in different conformations. We describe the assembly of CIIICIV from the CIII precursor to the final CIIICIV conformation, driven by the insertion of the N terminus of the assembly factor SCAF1 (ref. ) deep into CIII, while its C terminus is integrated into CIV. Our structures (which include CICIII and the respirasome) also confirm that SCAF1 is exclusively required for the assembly of CIIICIV and has no role in the assembly of the respirasome. We show that CIII is asymmetric due to the presence of only one copy of subunit 9, which straddles both monomers and prevents the attachment of a second copy of SCAF1 to CIII, explaining the presence of one copy of CIV in CIIICIV in mammals. Finally, we show that CIII and CIV gain catalytic advantage when assembled into the supercomplex and propose a role for CIIICIV in fine tuning the efficiency of electron transfer in the electron transport chain.

PubMed: 34616041
DOI: 10.1038/s41586-021-03927-z

実験手法

ELECTRON MICROSCOPY (3.3 Å)