7O1H

Hybrid-2R quadruplex-duplex with (-p-p-l) topology and 3 syn residues

Summary for 7O1H

• Entry DOI: 10.2210/pdb7o1h/pdb
• NMR Information: BMRB: 34615
• Descriptor: DNA (31-MER) (1 entity in total)
• Functional Keywords: quadruplex-duplex hybrid, hybrid topology, dna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 9873.08

Authors

Mohr, S.,Vianney, Y.M.,Weisz, K. (deposition date: 2021-03-29, release date: 2021-05-19, Last modification date: 2024-06-19)

Primary citation

Mohr, S.,Jana, J.,Vianney, Y.M.,Weisz, K.
Expanding the Topological Landscape by a G-Column Flip of a Parallel G-Quadruplex.
Chemistry, 27:10437-10447, 2021

PubMed Abstract: Canonical G-quadruplexes can adopt a variety of different topologies depending on the arrangement of propeller, lateral, or diagonal loops connecting the four G-columns. A novel intramolecular G-quadruplex structure is derived through inversion of the last G-tract of a three-layered parallel fold, associated with the transition of a single propeller into a lateral loop. The resulting (3+1) hybrid fold features three syn⋅anti⋅anti⋅anti G-tetrads with a 3'-terminal all-syn G-column. Although the ability of forming a duplex stem-loop between G-tracts seems beneficial for a propeller-to-lateral loop rearrangement, unmodified G-rich sequences resist folding into the new (3+1) topology. However, refolding can be driven by the incorporation of syn-favoring guanosine analogues into positions of the fourth G-stretch. The presented hybrid-type G-quadruplex structure as determined by NMR spectroscopy may provide for an additional scaffold in quadruplex-based technologies.

PubMed: 33955615
DOI: 10.1002/chem.202101181

Experimental method

SOLUTION NMR