7O1H

Hybrid-2R quadruplex-duplex with (-p-p-l) topology and 3 syn residues

7O1H の概要

• エントリーDOI: 10.2210/pdb7o1h/pdb
• NMR情報: BMRB: 34615
• 分子名称: DNA (31-MER) (1 entity in total)
• 機能のキーワード: quadruplex-duplex hybrid, hybrid topology, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9873.08

構造登録者

Mohr, S.,Vianney, Y.M.,Weisz, K. (登録日: 2021-03-29, 公開日: 2021-05-19, 最終更新日: 2024-06-19)

主引用文献

Mohr, S.,Jana, J.,Vianney, Y.M.,Weisz, K.
Expanding the Topological Landscape by a G-Column Flip of a Parallel G-Quadruplex.
Chemistry, 27:10437-10447, 2021

PubMed Abstract: Canonical G-quadruplexes can adopt a variety of different topologies depending on the arrangement of propeller, lateral, or diagonal loops connecting the four G-columns. A novel intramolecular G-quadruplex structure is derived through inversion of the last G-tract of a three-layered parallel fold, associated with the transition of a single propeller into a lateral loop. The resulting (3+1) hybrid fold features three syn⋅anti⋅anti⋅anti G-tetrads with a 3'-terminal all-syn G-column. Although the ability of forming a duplex stem-loop between G-tracts seems beneficial for a propeller-to-lateral loop rearrangement, unmodified G-rich sequences resist folding into the new (3+1) topology. However, refolding can be driven by the incorporation of syn-favoring guanosine analogues into positions of the fourth G-stretch. The presented hybrid-type G-quadruplex structure as determined by NMR spectroscopy may provide for an additional scaffold in quadruplex-based technologies.

PubMed: 33955615
DOI: 10.1002/chem.202101181

実験手法

SOLUTION NMR