7O0J

High resolution structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP)

7O0J の概要

• エントリーDOI: 10.2210/pdb7o0j/pdb
• 分子名称: Fatty acid-binding protein, liver (2 entities in total)
• 機能のキーワード: fatty acid binding protein, chicken liver bile acid binding protein, recombinant, lipid binding protein
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14513.65

構造登録者

Tassone, G.,Pozzi, C. (登録日: 2021-03-26, 公開日: 2021-05-12, 最終更新日: 2024-01-31)

主引用文献

Tassone, G.,Orlandini, M.,Olivucci, M.,Pozzi, C.
Validation of Recombinant Chicken Liver Bile Acid Binding Protein as a Tool for Cholic Acid Hosting.
Biomolecules, 11:-, 2021

PubMed Abstract: Bile acids (BAs) are hydroxylated steroids derived from cholesterol that act at the intestinal level to facilitate the absorption of several nutrients and also play a role as signaling molecules. In the liver of various vertebrates, the trafficking of BAs is mediated by bile acid-binding proteins (L-BABPs). The ability to host hydrophobic or amphipathic molecules makes BABPs suitable for the distribution of a variety of physiological and exogenous substances. Thus, BABPs have been proposed as drug carriers, and more recently, they have also been employed to develop innovative nanotechnology and biotechnology systems. Here, we report an efficient protocol for the production, purification, and crystallization of chicken liver BABP (cL-BABP). By means of target expression as His-tag cL-BABP, we obtained a large amount of pure and homogeneous proteins through a simple purification procedure relying on affinity chromatography. The recombinant cL-BABP showed a raised propensity to crystallize, allowing us to obtain its structure at high resolution and, in turn, assess the structural conservation of the recombinant cL-BABP with respect to the liver-extracted protein. The results support the use of recombinant cL-BABP for the development of drug carriers, nanotechnologies, and innovative synthetic photoswitch systems.

PubMed: 33925706
DOI: 10.3390/biom11050645

実験手法

X-RAY DIFFRACTION (1.4 Å)