7NYQ

Crystal structure of the Mei-P26 NHL domain

7NYQ の概要

• エントリーDOI: 10.2210/pdb7nyq/pdb
• 分子名称: Meiotic P26, isoform C (2 entities in total)
• 機能のキーワード: mei-p26, nhl, rna recognition, trim-nhl proteins, rna binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66587.99

構造登録者

Salerno-Kochan, A.,Gaik, M.,Medenbach, J.,Glatt, S. (登録日: 2021-03-23, 公開日: 2022-05-04, 最終更新日: 2024-10-16)

主引用文献

Salerno-Kochan, A.,Horn, A.,Ghosh, P.,Nithin, C.,Koscielniak, A.,Meindl, A.,Strauss, D.,Krutyholowa, R.,Rossbach, O.,Bujnicki, J.M.,Gaik, M.,Medenbach, J.,Glatt, S.
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26.
Life Sci Alliance, 5:-, 2022

PubMed Abstract: The TRIM-NHL protein Meiotic P26 (Mei-P26) acts as a regulator of cell fate in Its activity is critical for ovarian germline stem cell maintenance, differentiation of oocytes, and spermatogenesis. Mei-P26 functions as a post-transcriptional regulator of gene expression; however, the molecular details of how its NHL domain selectively recognizes and regulates its mRNA targets have remained elusive. Here, we present the crystal structure of the Mei-P26 NHL domain at 1.6 Å resolution and identify key amino acids that confer substrate specificity and distinguish Mei-P26 from closely related TRIM-NHL proteins. Furthermore, we identify mRNA targets of Mei-P26 in cultured cells and show that Mei-P26 can act as either a repressor or activator of gene expression on different RNA targets. Our work reveals the molecular basis of RNA recognition by Mei-P26 and the fundamental functional differences between otherwise very similar TRIM-NHL proteins.

PubMed: 35512835
DOI: 10.26508/lsa.202201418

実験手法

X-RAY DIFFRACTION (1.6 Å)