7NUM

Rhinovirus-14 ICAM-1 empty particle at pH 6.2

7NUM の概要

• エントリーDOI: 10.2210/pdb7num/pdb
• 関連するPDBエントリー: 7BG6 7BG7
• EMDBエントリー: 12171 12172 12595
• 分子名称: Genome polyprotein, P1 (3 entities in total)
• 機能のキーワード: rhinovirus 14, rv14, hrv14, acidification, ph 6.2, genome release, emptu, icam-1, virus
• 由来する生物種: Human rhinovirus 14 (HRV-14); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87298.66

構造登録者

Hrebik, D.,Plevka, P. (登録日: 2021-03-12, 公開日: 2021-05-19, 最終更新日: 2024-07-10)

主引用文献

Hrebik, D.,Fuzik, T.,Gondova, M.,Smerdova, L.,Adamopoulos, A.,Sedo, O.,Zdrahal, Z.,Plevka, P.
ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Most rhinoviruses, which are the leading cause of the common cold, utilize intercellular adhesion molecule-1 (ICAM-1) as a receptor to infect cells. To release their genomes, rhinoviruses convert to activated particles that contain pores in the capsid, lack minor capsid protein VP4, and have an altered genome organization. The binding of rhinoviruses to ICAM-1 promotes virus activation; however, the molecular details of the process remain unknown. Here, we present the structures of virion of rhinovirus 14 and its complex with ICAM-1 determined to resolutions of 2.6 and 2.4 Å, respectively. The cryo-electron microscopy reconstruction of rhinovirus 14 virions contains the resolved density of octanucleotide segments from the RNA genome that interact with VP2 subunits. We show that the binding of ICAM-1 to rhinovirus 14 is required to prime the virus for activation and genome release at acidic pH. Formation of the rhinovirus 14-ICAM-1 complex induces conformational changes to the rhinovirus 14 capsid, including translocation of the C termini of VP4 subunits, which become poised for release through pores that open in the capsids of activated particles. VP4 subunits with altered conformation block the RNA-VP2 interactions and expose patches of positively charged residues. The conformational changes to the capsid induce the redistribution of the virus genome by altering the capsid-RNA interactions. The restructuring of the rhinovirus 14 capsid and genome prepares the virions for conversion to activated particles. The high-resolution structure of rhinovirus 14 in complex with ICAM-1 explains how the binding of uncoating receptors enables enterovirus genome release.

PubMed: 33947819
DOI: 10.1073/pnas.2024251118

実験手法

ELECTRON MICROSCOPY (3.9 Å)