7NO1

Structure of the mature RSV CA lattice: T=3 CA icosahedron

7NO1 の概要

• エントリーDOI: 10.2210/pdb7no1/pdb
• EMDBエントリー: 12486
• 分子名称: Capsid protein p27, alternate cleaved 1 (1 entity in total)
• 機能のキーワード: retrovirus, rous sarcoma virus, capsid protein, ip6, viral protein
• 由来する生物種: Rous sarcoma virus (strain Prague C) (RSV-PrC)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 74320.78

構造登録者

Obr, M.,Ricana, C.L.,Nikulin, N.,Feathers, J.-P.R.,Klanschnig, M.,Thader, A.,Johnson, M.C.,Vogt, V.M.,Schur, F.K.M.,Dick, R.A. (登録日: 2021-02-25, 公開日: 2021-04-21, 最終更新日: 2024-07-10)

主引用文献

Obr, M.,Ricana, C.L.,Nikulin, N.,Feathers, J.R.,Klanschnig, M.,Thader, A.,Johnson, M.C.,Vogt, V.M.,Schur, F.K.M.,Dick, R.A.
Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer.
Nat Commun, 12:3226-3226, 2021

PubMed Abstract: Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a retrovirus from a different genus. IP6 is ~100-fold more potent at promoting RSV mature capsid protein (CA) assembly than observed for HIV-1 and removal of IP6 in cells reduces infectivity by 100-fold. Here, visualized by cryo-electron tomography and subtomogram averaging, mature capsid-like particles show an IP6-like density in the CA hexamer, coordinated by rings of six lysines and six arginines. Phosphate and IP6 have opposing effects on CA in vitro assembly, inducing formation of T = 1 icosahedrons and tubes, respectively, implying that phosphate promotes pentamer and IP6 hexamer formation. Subtomogram averaging and classification optimized for analysis of pleomorphic retrovirus particles reveal that the heterogeneity of mature RSV CA polyhedrons results from an unexpected, intrinsic CA hexamer flexibility. In contrast, the CA pentamer forms rigid units organizing the local architecture. These different features of hexamers and pentamers determine the structural mechanism to form CA polyhedrons of variable shape in mature RSV particles.

PubMed: 34050170
DOI: 10.1038/s41467-021-23506-0

実験手法

ELECTRON MICROSCOPY (7.6 Å)