7NIB

Wzc-K540M-4YE C1

7NIB の概要

• エントリーDOI: 10.2210/pdb7nib/pdb
• EMDBエントリー: 12353
• 分子名称: Tyrosine-protein kinase (1 entity in total)
• 機能のキーワード: wzc, regulator, capsular polysaccharide synthesis and transport, gram-negative pathogens, carbohydrate
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 643480.87

構造登録者

Naismith, J.H.,Liu, J.W.,Yang, Y. (登録日: 2021-02-11, 公開日: 2021-08-25, 最終更新日: 2025-07-09)

主引用文献

Yang, Y.,Liu, J.,Clarke, B.R.,Seidel, L.,Bolla, J.R.,Ward, P.N.,Zhang, P.,Robinson, C.V.,Whitfield, C.,Naismith, J.H.
The molecular basis of regulation of bacterial capsule assembly by Wzc.
Nat Commun, 12:4349-4349, 2021

PubMed Abstract: Bacterial extracellular polysaccharides (EPSs) play critical roles in virulence. Many bacteria assemble EPSs via a multi-protein "Wzx-Wzy" system, involving glycan polymerization at the outer face of the cytoplasmic/inner membrane. Gram-negative species couple polymerization with translocation across the periplasm and outer membrane and the master regulator of the system is the tyrosine autokinase, Wzc. This near atomic cryo-EM structure of dephosphorylated Wzc from E. coli shows an octameric assembly with a large central cavity formed by transmembrane helices. The tyrosine autokinase domain forms the cytoplasm region, while the periplasmic region contains small folded motifs and helical bundles. The helical bundles are essential for function, most likely through interaction with the outer membrane translocon, Wza. Autophosphorylation of the tyrosine-rich C-terminus of Wzc results in disassembly of the octamer into multiply phosphorylated monomers. We propose that the cycling between phosphorylated monomer and dephosphorylated octamer regulates glycan polymerization and translocation.

PubMed: 34272394
DOI: 10.1038/s41467-021-24652-1

実験手法

ELECTRON MICROSCOPY (3.51 Å)