7NF9
N-terminal C2H2 Zn-finger domain of Clamp
Summary for 7NF9
| Entry DOI | 10.2210/pdb7nf9/pdb |
| NMR Information | BMRB: 34600 |
| Descriptor | Chromatin-linked adaptor for MSL proteins, isoform A, ZINC ION (2 entities in total) |
| Functional Keywords | dosage compensation zinc finger, gene regulation |
| Biological source | Drosophila melanogaster (Fruit fly) |
| Total number of polymer chains | 1 |
| Total formula weight | 7477.82 |
| Authors | Mariasina, S.S.,Bonchuk, A.N.,Tikhonova, E.A.,Efimov, S.V.,Maksimenko, O.G.,Georgiev, P.G.,Polshakov, V.I. (deposition date: 2021-02-05, release date: 2021-12-29, Last modification date: 2024-06-19) |
| Primary citation | Tikhonova, E.,Mariasina, S.,Efimov, S.,Polshakov, V.,Maksimenko, O.,Georgiev, P.,Bonchuk, A. Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila. Nucleic Acids Res., 50:6521-6531, 2022 Cited by PubMed Abstract: Transcriptional regulators select their targets from a large pool of similar genomic sites. The binding of the Drosophila dosage compensation complex (DCC) exclusively to the male X chromosome provides insight into binding site selectivity rules. Previous studies showed that the male-specific organizer of the complex, MSL2, and ubiquitous DNA-binding protein CLAMP directly interact and play an important role in the specificity of X chromosome binding. Here, we studied the highly specific interaction between the intrinsically disordered region of MSL2 and the N-terminal zinc-finger C2H2-type (C2H2) domain of CLAMP. We obtained the NMR structure of the CLAMP N-terminal C2H2 zinc finger, which has a classic C2H2 zinc-finger fold with a rather unusual distribution of residues typically used in DNA recognition. Substitutions of residues in this C2H2 domain had the same effect on the viability of males and females, suggesting that it plays a general role in CLAMP activity. The N-terminal C2H2 domain of CLAMP is highly conserved in insects. However, the MSL2 region involved in the interaction is conserved only within the Drosophila genus, suggesting that this interaction emerged during the evolution of a mechanism for the specific recruitment of the DCC on the male X chromosome in Drosophilidae. PubMed: 35648444DOI: 10.1093/nar/gkac455 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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