7NDE

Trichoderma parareesei PL7A beta-glucuronan lyase

Summary for 7NDE

Entry DOI	10.2210/pdb7nde/pdb
Descriptor	Glucuronan lyase, 2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (4 entities in total)
Functional Keywords	glucuronan lyase, b-jelly roll, lyase
Biological source	Trichoderma parareesei
Total number of polymer chains	1
Total formula weight	25997.53
Authors	Fredslund, F.,Welner, D.H.,Wilkens, C. (deposition date: 2021-02-01, release date: 2022-03-02, Last modification date: 2024-10-16)
Primary citation	Vuillemin, M.,Pilgaard, B.,Kiehn, E.,Fredslund, F.,Welner, D.H.,Meyer, A.S.,Aachmann, F.L.,Wilkens, C. Glucuronan lyases from family PL7 use a Tyr/Tyr syn beta-elimination catalytic mechanism for glucuronan breakdown. Chem.Commun.(Camb.), 60:440-443, 2024 Cited by PubMed Abstract: TpPL7A and TpPL7B, members of CAZy family PL7, act as β-glucuronan lyases. TpPL7A diverges by lacking the catalytic histidine, identified as the Brønsted base in PL7 alginate lyases. Our research, including TpPL7A's crystal structure, and mutagenesis studies, reveals a shared -β-elimination mechanism with a single tyrosine serving as both base and acid catalyst. This mechanism may extend to subfamily PL7_4 glucuronan lyases. PubMed: 38087900 DOI: 10.1039/d3cc04256a PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.45 Å)

Structure validation