Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

7NDE

Trichoderma parareesei PL7A beta-glucuronan lyase

Summary for 7NDE
Entry DOI10.2210/pdb7nde/pdb
DescriptorGlucuronan lyase, 2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (4 entities in total)
Functional Keywordsglucuronan lyase, b-jelly roll, lyase
Biological sourceTrichoderma parareesei
Total number of polymer chains1
Total formula weight25997.53
Authors
Fredslund, F.,Welner, D.H.,Wilkens, C. (deposition date: 2021-02-01, release date: 2022-03-02, Last modification date: 2024-10-16)
Primary citationVuillemin, M.,Pilgaard, B.,Kiehn, E.,Fredslund, F.,Welner, D.H.,Meyer, A.S.,Aachmann, F.L.,Wilkens, C.
Glucuronan lyases from family PL7 use a Tyr/Tyr syn beta-elimination catalytic mechanism for glucuronan breakdown.
Chem.Commun.(Camb.), 60:440-443, 2024
Cited by
PubMed Abstract: TpPL7A and TpPL7B, members of CAZy family PL7, act as β-glucuronan lyases. TpPL7A diverges by lacking the catalytic histidine, identified as the Brønsted base in PL7 alginate lyases. Our research, including TpPL7A's crystal structure, and mutagenesis studies, reveals a shared -β-elimination mechanism with a single tyrosine serving as both base and acid catalyst. This mechanism may extend to subfamily PL7_4 glucuronan lyases.
PubMed: 38087900
DOI: 10.1039/d3cc04256a
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.45 Å)
Structure validation

239149

数据于2025-07-23公开中

PDB statisticsPDBj update infoContact PDBjnumon