7NDE

Trichoderma parareesei PL7A beta-glucuronan lyase

7NDE の概要

• エントリーDOI: 10.2210/pdb7nde/pdb
• 分子名称: Glucuronan lyase, 2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: glucuronan lyase, b-jelly roll, lyase
• 由来する生物種: Trichoderma parareesei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25997.53

構造登録者

Fredslund, F.,Welner, D.H.,Wilkens, C. (登録日: 2021-02-01, 公開日: 2022-03-02, 最終更新日: 2024-10-16)

主引用文献

Vuillemin, M.,Pilgaard, B.,Kiehn, E.,Fredslund, F.,Welner, D.H.,Meyer, A.S.,Aachmann, F.L.,Wilkens, C.
Glucuronan lyases from family PL7 use a Tyr/Tyr syn beta-elimination catalytic mechanism for glucuronan breakdown.
Chem.Commun.(Camb.), 60:440-443, 2024

PubMed Abstract: TpPL7A and TpPL7B, members of CAZy family PL7, act as β-glucuronan lyases. TpPL7A diverges by lacking the catalytic histidine, identified as the Brønsted base in PL7 alginate lyases. Our research, including TpPL7A's crystal structure, and mutagenesis studies, reveals a shared -β-elimination mechanism with a single tyrosine serving as both base and acid catalyst. This mechanism may extend to subfamily PL7_4 glucuronan lyases.

PubMed: 38087900
DOI: 10.1039/d3cc04256a

実験手法

X-RAY DIFFRACTION (1.45 Å)