7N8O

High-resolution structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803

Summary for 7N8O

• Entry DOI: 10.2210/pdb7n8o/pdb
• EMDB information: 24239
• Descriptor: Photosystem II protein D1 2, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (38 entities in total)
• Functional Keywords: photosystem ii, cyanobacteria, synechocystis 6803, mesophile, cyanoq, photosynthesis
• Biological source: Synechocystis sp. (strain PCC 6803 / Kazusa); More
• Total number of polymer chains: 42
• Total formula weight: 808199.57

Authors

Gisriel, C.J.,Brudvig, G.W. (deposition date: 2021-06-15, release date: 2021-12-29, Last modification date: 2024-11-13)

Primary citation

Gisriel, C.J.,Wang, J.,Liu, J.,Flesher, D.A.,Reiss, K.M.,Huang, H.L.,Yang, K.R.,Armstrong, W.H.,Gunner, M.R.,Batista, V.S.,Debus, R.J.,Brudvig, G.W.
High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Photosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium sp. PCC 6803 has provided insights into the mechanism of water oxidation; however, the lack of a high-resolution structure of oxygen-evolving PSII from this organism has limited the interpretation of biophysical data to models based on structures of thermophilic cyanobacterial PSII. Here, we report the cryo-electron microscopy structure of PSII from sp. PCC 6803 at 1.93-Å resolution. A number of differences are observed relative to thermophilic PSII structures, including the following: the extrinsic subunit PsbQ is maintained, the C terminus of the D1 subunit is flexible, some waters near the active site are partially occupied, and differences in the PsbV subunit block the Large (O1) water channel. These features strongly influence the structural picture of PSII, especially as it pertains to the mechanism of water oxidation.

PubMed: 34937700
DOI: 10.1073/pnas.2116765118

Experimental method

ELECTRON MICROSCOPY (1.93 Å)