7N6I

ATP-bound TnsC-TniQ complex from ShCAST system

7N6I の概要

• エントリーDOI: 10.2210/pdb7n6i/pdb
• EMDBエントリー: 23720 23721 23722 23723 23724 23725 23726
• 分子名称: TniQ (Homology model), TnsC, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), ... (6 entities in total)
• 機能のキーワード: crispr, transposition, aaa+ atpase, tn7, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Scytonema hofmannii; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 303705.70

構造登録者

Park, J.,Tsai, A.W.L.,Mehrotra, E.,Kellogg, E.H. (登録日: 2021-06-08, 公開日: 2021-07-28, 最終更新日: 2024-05-29)

主引用文献

Park, J.U.,Tsai, A.W.,Mehrotra, E.,Petassi, M.T.,Hsieh, S.C.,Ke, A.,Peters, J.E.,Kellogg, E.H.
Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373:768-774, 2021

PubMed Abstract: CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. We used cryo-electron microscopy (cryo-EM) to define the mechanism that underlies this process by characterizing the transposition regulator, TnsC, from a type V-K CRISPR-transposase system. In this scenario, polymerization of adenosine triphosphate-bound TnsC helical filaments could explain how polarity information is passed to the transposase. TniQ caps the TnsC filament, representing a universal mechanism for target information transfer in Tn7/Tn7-like elements. Transposase-driven disassembly establishes delivery of the element only to unused protospacers. Finally, TnsC transitions to define the fixed point of insertion, as revealed by structures with the transition state mimic ADP•AlF These mechanistic findings provide the underpinnings for engineering CRISPR-associated transposition systems for research and therapeutic applications.

PubMed: 34385391
DOI: 10.1126/science.abi8976

実験手法

ELECTRON MICROSCOPY (3.9 Å)