7N61

structure of C2 projections and MIPs

これはPDB形式変換不可エントリーです。

7N61 の概要

• エントリーDOI: 10.2210/pdb7n61/pdb
• EMDBエントリー: 24191 24207
• 分子名称: Flagellar WD repeat-containing protein Pf20, Unassigned protein-3, Unassigned protein-4, ... (21 entities in total)
• 機能のキーワード: central pair apparatus, c2 projection, kinesin motor protein, structural protein
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 139
• 化学式量合計: 7556779.60

構造登録者

Han, L.,Zhang, K. (登録日: 2021-06-07, 公開日: 2022-05-18, 最終更新日: 2024-05-29)

主引用文献

Han, L.,Rao, Q.,Yang, R.,Wang, Y.,Chai, P.,Xiong, Y.,Zhang, K.
Cryo-EM structure of an active central apparatus.
Nat.Struct.Mol.Biol., 29:472-482, 2022

PubMed Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.

PubMed: 35578022
DOI: 10.1038/s41594-022-00769-9

実験手法

ELECTRON MICROSCOPY (3.5 Å)