7MWI

Crystal structure of human BAZ2A

7MWI の概要

• エントリーDOI: 10.2210/pdb7mwi/pdb
• 分子名称: Bromodomain adjacent to zinc finger domain protein 2A, UNKNOWN ATOM OR ION (3 entities in total)
• 機能のキーワード: mbd, tam, structural genomics, structural genomics consortium, sgc, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13975.93

構造登録者

Liu, K.,Dong, A.,Li, Y.,Loppnau, P.,Edwards, A.M.,Arrowsmith, C.H.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2021-05-17, 公開日: 2021-12-29, 最終更新日: 2023-10-18)

主引用文献

Chen, S.,Zhou, M.,Dong, A.,Loppnau, P.,Wang, M.,Min, J.,Liu, K.
Structural basis of the TAM domain of BAZ2A in binding to DNA or RNA independent of methylation status.
J.Biol.Chem., 297:101351-101351, 2021

PubMed Abstract: Bromodomain adjacent to zinc finger domain protein 2A (BAZ2A) (also called transcription termination factor-1 interacting protein 5), a key component of the nucleolar remodeling complex, recruits the nucleolar remodeling complex to ribosomal RNA genes, leading to their transcriptional repression. In addition to its tandem plant homeodomain-bromodomain that is involved in binding to acetylated histone H4, BAZ2A also contains a methyl-CpG-binding domain (MBD)-like Tip5/ARBP/MBD (TAM) domain that shares sequence homology with the MBD. In contrast with the methyl-CpG-binding ability of the canonical MBD, the BAZ2A TAM domain has been shown to bind to promoter-associated RNAs of ribosomal RNA genes and promoter DNAs of other genes independent of DNA methylation. Nevertheless, how the TAM domain binds to RNA/DNA mechanistically remains elusive. Here, we characterized the DNA-/RNA-binding basis of the BAZ2A TAM domain by EMSAs, isothermal titration calorimetry binding assays, mutagenesis analysis, and X-ray crystallography. Our results showed that the TAM domain of BAZ2A selectively binds to dsDNA and dsRNA and that it binds to the backbone of dsDNA in a sequence nonspecific manner, which is distinct from the base-specific binding of the canonical MBD. Thus, our results explain why the TAM domain of BAZ2A does not specifically bind to mCG or TG dsDNA like the canonical MBD and also provide insights for further biological study of BAZ2A acting as a transcription factor in the future.

PubMed: 34715126
DOI: 10.1016/j.jbc.2021.101351

実験手法

X-RAY DIFFRACTION (1.8 Å)