7MUC

Legionella pneumophila Dot/Icm T4SS C1 Reconstruction

これはPDB形式変換不可エントリーです。

7MUC の概要

• エントリーDOI: 10.2210/pdb7muc/pdb
• EMDBエントリー: 24004
• 分子名称: DotC, Unknown protein fragment, DotD, ... (11 entities in total)
• 機能のキーワード: legionella, dot/icm, secretion, t4ss, translocase
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 189
• 化学式量合計: 5836685.32

構造登録者

Sheedlo, M.J.,Durie, C.L.,Swanson, M.,Lacy, D.B.,Ohi, M.D. (登録日: 2021-05-14, 公開日: 2021-10-06, 最終更新日: 2022-04-20)

主引用文献

Sheedlo, M.J.,Durie, C.L.,Chung, J.M.,Chang, L.,Roberts, J.,Swanson, M.,Lacy, D.B.,Ohi, M.D.
Cryo-EM reveals new species-specific proteins and symmetry elements in the Legionella pneumophila Dot/Icm T4SS.
Elife, 10:-, 2021

PubMed Abstract: is an opportunistic pathogen that causes the potentially fatal pneumonia known as Legionnaires' disease. The pathology associated with infection depends on bacterial delivery of effector proteins into the host via the membrane spanning Dot/Icm type IV secretion system (T4SS). We have determined sub-3.0 Å resolution maps of the Dot/Icm T4SS core complex by single particle cryo-EM. The high-resolution structural analysis has allowed us to identify proteins encoded outside the Dot/Icm genetic locus that contribute to the core T4SS structure. We can also now define two distinct areas of symmetry mismatch, one that connects the C18 periplasmic ring (PR) and the C13 outer membrane cap (OMC) and one that connects the C13 OMC with a 16-fold symmetric dome. Unexpectedly, the connection between the PR and OMC is DotH, with five copies sandwiched between the OMC and PR to accommodate the symmetry mismatch. Finally, we observe multiple conformations in the reconstructions that indicate flexibility within the structure.

PubMed: 34519271
DOI: 10.7554/eLife.70427

実験手法

ELECTRON MICROSCOPY (3.8 Å)