7MT9

Rhodopsin kinase (GRK1) in complex with rhodopsin

7MT9 の概要

• エントリーDOI: 10.2210/pdb7mt9/pdb
• EMDBエントリー: 23978
• 分子名称: Rhodopsin kinase GRK1, Rhodopsin, SANGIVAMYCIN, ... (4 entities in total)
• 機能のキーワード: gpcr, signal desensitization, kinase, membrane protein, membrane protein-signaling protein complex, membrane protein/signaling protein
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101055.11

構造登録者

Chen, Q.,Chen, C.-L.,Tesmer, J.J.G. (登録日: 2021-05-13, 公開日: 2021-07-07, 最終更新日: 2021-08-25)

主引用文献

Chen, Q.,Plasencia, M.,Li, Z.,Mukherjee, S.,Patra, D.,Chen, C.L.,Klose, T.,Yao, X.Q.,Kossiakoff, A.A.,Chang, L.,Andrews, P.C.,Tesmer, J.J.G.
Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Nature, 595:600-605, 2021

PubMed Abstract: G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization. Although it is unclear how GRKs recognize these receptors, a conserved region at the GRK N terminus is essential for this process. Here we report a series of cryo-electron microscopy single-particle reconstructions of light-activated rhodopsin (Rho*) bound to rhodopsin kinase (GRK1), wherein the N terminus of GRK1 forms a helix that docks into the open cytoplasmic cleft of Rho*. The helix also packs against the GRK1 kinase domain and stabilizes it in an active configuration. The complex is further stabilized by electrostatic interactions between basic residues that are conserved in most GPCRs and acidic residues that are conserved in GRKs. We did not observe any density for the regulator of G-protein signalling homology domain of GRK1 or the C terminus of rhodopsin. Crosslinking with mass spectrometry analysis confirmed these results and revealed dynamic behaviour in receptor-bound GRK1 that would allow the phosphorylation of multiple sites in the receptor tail. We have identified GRK1 residues whose mutation augments kinase activity and crosslinking with Rho*, as well as residues that are involved in activation by acidic phospholipids. From these data, we present a general model for how a small family of protein kinases can recognize and be activated by hundreds of different GPCRs.

PubMed: 34262173
DOI: 10.1038/s41586-021-03721-x

実験手法

ELECTRON MICROSCOPY (7 Å)