7MSY

Structure of CalU17 from the Calicheamicin Biosynthesis Pathway of Micromonospora echinospora

Summary for 7MSY

• Entry DOI: 10.2210/pdb7msy/pdb
• Related: 6VQP 7ML6
• Descriptor: CalU17, CALCIUM ION, GLYCEROL, ... (6 entities in total)
• Functional Keywords: enediyne biosynthetic pathway, calicheamicin biosynthesis, unknown function
• Biological source: Micromonospora echinospora (Micromonospora purpurea)
• Total number of polymer chains: 1
• Total formula weight: 35976.91

Authors

Kosgei, A.J.,Miller, M.D.,Xu, W.,Van Lanen, S.G.,Thorson, J.S.,Phillips Jr., G.N. (deposition date: 2021-05-12, release date: 2021-07-28, Last modification date: 2023-10-18)

Primary citation

Kosgei, A.J.,Miller, M.D.,Bhardwaj, M.,Xu, W.,Thorson, J.S.,Van Lanen, S.G.,Phillips Jr., G.N.
The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina.
Acta Crystallogr.,Sect.F, 78:1-7, 2022

PubMed Abstract: Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

PubMed: 34981769
DOI: 10.1107/S2053230X21012322

Experimental method

X-RAY DIFFRACTION (2.21 Å)