7MSF

MS2 PROTEIN CAPSID/RNA COMPLEX

7MSF の概要

• エントリーDOI: 10.2210/pdb7msf/pdb
• 分子名称: 5'-R(*UP*CP*GP*CP*CP*AP*AP*CP*AP*GP*GP*CP*GP*G)-3', MS2 PROTEIN CAPSID (3 entities in total)
• 機能のキーワード: ccapsid protein ms2-rna aptamer complex, rna-protein complex, rna stem loop, bacteriophage ms2, icosahedral virus, virus-rna complex, virus/rna
• 由来する生物種: Enterobacterio phage MS2; 詳細
• 細胞内の位置: Virion (Potential): P03612
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 50216.92

構造登録者

Rowsell, S.,Stonehouse, N.J.,Convery, M.A.,Adams, C.J.,Ellington, A.D.,Hirao, I.,Peabody, D.S.,Stockley, P.G.,Phillips, S.E.V. (登録日: 1998-05-20, 公開日: 1998-11-11, 最終更新日: 2023-08-02)

主引用文献

Rowsell, S.,Stonehouse, N.J.,Convery, M.A.,Adams, C.J.,Ellington, A.D.,Hirao, I.,Peabody, D.S.,Stockley, P.G.,Phillips, S.E.
Crystal structures of a series of RNA aptamers complexed to the same protein target.
Nat.Struct.Biol., 5:970-975, 1998

PubMed Abstract: We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.

PubMed: 9808042
DOI: 10.1038/2946

実験手法

X-RAY DIFFRACTION (2.8 Å)