7MRK

Chicken CNTN4 APP complex

7MRK の概要

• エントリーDOI: 10.2210/pdb7mrk/pdb
• 関連するPDBエントリー: 7MQY
• 分子名称: Amyloid-beta A4 protein, Contactin-4, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: ig superfamily, amyloid precursor protein, cell adhesion
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108387.56

構造登録者

Bouyain, S.,Karuppan, S.J. (登録日: 2021-05-07, 公開日: 2022-01-05, 最終更新日: 2024-11-06)

主引用文献

Karuppan, S.J.,Vogt, A.,Fischer, Z.,Ladutska, A.,Swiastyn, J.,McGraw, H.F.,Bouyain, S.
Members of the vertebrate contactin and amyloid precursor protein families interact through a conserved interface.
J.Biol.Chem., 298:101541-101541, 2021

PubMed Abstract: Contactins (CNTNs) are neural cell adhesion molecules that encode axon-target specificity during the patterning of the vertebrate visual and olfactory systems. Because CNTNs are tethered to the plasma membrane by a glycosylphosphatidylinositol anchor, they lack an intracellular region to communicate across the membrane. Instead, they form coreceptor complexes with distinct transmembrane proteins to transmit signals inside the cell. In particular, a complex of CNTN4 and amyloid precursor protein (APP) is known to guide the assembly of specific circuits in the visual system. Here, using in situ hybridization in zebrafish embryos, we show that CNTN4, CNTN5, and the APP homologs, amyloid beta precursor like protein 1 and amyloid beta precursor like protein 2, are expressed in olfactory pits, suggesting that these receptors may also function together in the organization of olfactory tissues. Furthermore, we use biochemical and structural approaches to characterize interactions between members of these two receptor families. In particular, APP and amyloid beta precursor like protein 1 interact with CNTN3-5, whereas amyloid beta precursor like protein 2 only binds to CNTN4 and CNTN5. Finally, structural analyses of five CNTN-amyloid pairs indicate that these proteins interact through a conserved interface involving the second fibronectin type III repeat of CNTNs and the copper-binding domain of amyloid proteins. Overall, this work sets the stage for analyzing CNTN-amyloid-mediated connectivity in vertebrate sensory circuits.

PubMed: 34958801
DOI: 10.1016/j.jbc.2021.101541

実験手法

X-RAY DIFFRACTION (2.05 Å)