7ML6

Structure of CalU17 from the Calicheamicin Biosynthesis Pathway of Micromonospora echinospora

7ML6 の概要

• エントリーDOI: 10.2210/pdb7ml6/pdb
• 関連するPDBエントリー: 6VQP 7MSY
• 分子名称: CalU17, GLYCEROL (3 entities in total)
• 機能のキーワード: enediyne biosynthetic pathway, calicheamicin biosynthesis, unknown function
• 由来する生物種: Micromonospora echinospora (Micromonospora purpurea)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35473.24

構造登録者

Kosgei, A.J.,Miller, M.D.,Xu, W.,Van Lanen, S.G.,Thorson, J.S.,Phillips Jr., G.N. (登録日: 2021-04-27, 公開日: 2021-07-28, 最終更新日: 2023-10-18)

主引用文献

Kosgei, A.J.,Miller, M.D.,Bhardwaj, M.,Xu, W.,Thorson, J.S.,Van Lanen, S.G.,Phillips Jr., G.N.
The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina.
Acta Crystallogr.,Sect.F, 78:1-7, 2022

PubMed Abstract: Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

PubMed: 34981769
DOI: 10.1107/S2053230X21012322

実験手法

X-RAY DIFFRACTION (2.1 Å)