7MIL

Mouse TRPV3 in MSP2N2 nanodiscs, sensitized state at 42 degrees Celsius

7MIL の概要

• エントリーDOI: 10.2210/pdb7mil/pdb
• EMDBエントリー: 23855
• 分子名称: Transient receptor potential cation channel subfamily V member 3, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, SODIUM ION (3 entities in total)
• 機能のキーワード: transient receptor potential v family member 3, trp channel, trpv3, sensitized state at 42 degrees celsius, msp2n2, membrane protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 376626.38

構造登録者

Nadezhdin, K.D.,Neuberger, A.,Sobolevsky, A.I. (登録日: 2021-04-17, 公開日: 2021-07-21, 最終更新日: 2024-10-16)

主引用文献

Nadezhdin, K.D.,Neuberger, A.,Trofimov, Y.A.,Krylov, N.A.,Sinica, V.,Kupko, N.,Vlachova, V.,Zakharian, E.,Efremov, R.G.,Sobolevsky, A.I.
Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel.
Nat.Struct.Mol.Biol., 28:564-572, 2021

PubMed Abstract: Numerous physiological functions rely on distinguishing temperature through temperature-sensitive transient receptor potential channels (thermo-TRPs). Although the function of thermo-TRPs has been studied extensively, structural determination of their heat- and cold-activated states has remained a challenge. Here, we present cryo-EM structures of the nanodisc-reconstituted wild-type mouse TRPV3 in three distinct conformations: closed, heat-activated sensitized and open states. The heat-induced transformations of TRPV3 are accompanied by changes in the secondary structure of the S2-S3 linker and the N and C termini and represent a conformational wave that links these parts of the protein to a lipid occupying the vanilloid binding site. State-dependent differences in the behavior of bound lipids suggest their active role in thermo-TRP temperature-dependent gating. Our structural data, supported by physiological recordings and molecular dynamics simulations, provide an insight for understanding the molecular mechanism of temperature sensing.

PubMed: 34239124
DOI: 10.1038/s41594-021-00615-4

実験手法

ELECTRON MICROSCOPY (3.86 Å)