7MHT

CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX

7MHT の概要

• エントリーDOI: 10.2210/pdb7mht/pdb
• 分子名称: 5'-D(P*GP*TP*CP*AP*GP*CP*GP*CP*AP*TP*GP*G)-3', 5'-D(P*CP*CP*AP*TP*GP*AP*GP*CP*TP*GP*AP*C)-3', CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI, ... (5 entities in total)
• 機能のキーワード: transferase, methyltransferase, restriction system, complex (methyltransferase- dna), transferase-dna complex, transferase/dna
• 由来する生物種: Haemophilus haemolyticus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44777.43

構造登録者

O'Gara, M.,Horton, J.R.,Roberts, R.J.,Cheng, X. (登録日: 1998-08-05, 公開日: 1998-11-30, 最終更新日: 2023-09-20)

主引用文献

O'Gara, M.,Horton, J.R.,Roberts, R.J.,Cheng, X.
Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base.
Nat.Struct.Biol., 5:872-877, 1998

PubMed Abstract: Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.

PubMed: 9783745
DOI: 10.1038/2312

実験手法

X-RAY DIFFRACTION (2.87 Å)