7MH2

T4GALA Engineered Protein Nanocage

Summary for 7MH2

• Entry DOI: 10.2210/pdb7mh2/pdb
• EMDB information: 23834
• Descriptor: T4GALA Engineered Protein Nanocage (1 entity in total)
• Functional Keywords: encapsulin, nanocage, nanocompartment, virus like particle
• Biological source: Quasibacillus thermotolerans
• Total number of polymer chains: 4
• Total formula weight: 141162.61

Authors

Andreas, M.P.,Jones, J.A.,Cristie-David, A.S.,Giessen, T.W. (deposition date: 2021-04-14, release date: 2021-09-29, Last modification date: 2024-05-29)

Primary citation

Jones, J.A.,Cristie-David, A.S.,Andreas, M.P.,Giessen, T.W.
Triggered Reversible Disassembly of an Engineered Protein Nanocage*.
Angew.Chem.Int.Ed.Engl., 60:25034-25041, 2021

PubMed Abstract: Protein nanocages play crucial roles in sub-cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug delivery, and bionanotechnology. The ability to control their assembly state under physiological conditions would further expand their practical utility. To gain such control, we introduced a peptide capable of triggering conformational change at a key structural position in the largest known encapsulin nanocompartment. We report the structure of the resulting engineered nanocage and demonstrate its ability to disassemble and reassemble on demand under physiological conditions. We demonstrate its capacity for in vivo encapsulation of proteins of choice while also demonstrating in vitro cargo loading capabilities. Our results represent a functionally robust addition to the nanocage toolbox and a novel approach for controlling protein nanocage disassembly and reassembly under mild conditions.

PubMed: 34532937
DOI: 10.1002/anie.202110318

Experimental method

ELECTRON MICROSCOPY (3.57 Å)