7MFX

The Crystal Structure of Q108K:K40H:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with FR1 Chromophore Showing Excited State Intermolecular Proton Transfer

7MFX の概要

• エントリーDOI: 10.2210/pdb7mfx/pdb
• 分子名称: Retinol-binding protein 2, (4aP)-N,N-diethyl-9,9-dimethyl-7-[(1E)-prop-1-en-1-yl]-9H-fluoren-2-amine, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: domain swapped trimer, ilbp, lipid binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 63599.91

構造登録者

Ghanbarpour, A.,Geiger, J. (登録日: 2021-04-11, 公開日: 2022-01-12, 最終更新日: 2024-11-06)

主引用文献

Santos, E.M.,Sheng, W.,Esmatpour Salmani, R.,Tahmasebi Nick, S.,Ghanbarpour, A.,Gholami, H.,Vasileiou, C.,Geiger, J.H.,Borhan, B.
Design of Large Stokes Shift Fluorescent Proteins Based on Excited State Proton Transfer of an Engineered Photobase.
J.Am.Chem.Soc., 143:15091-15102, 2021

PubMed Abstract: The incredible potential for fluorescent proteins to revolutionize biology has inspired the development of a variety of design strategies to address an equally broad range of photophysical characteristics, depending on potential applications. Of these, fluorescent proteins that simultaneously exhibit high quantum yield, red-shifted emission, and wide separation between excitation and emission wavelengths (Large Stokes Shift, LSS) are rare. The pursuit of LSS systems has led to the formation of a complex, obtained from the marriage of a rationally engineered protein (human cellular retinol binding protein II, hCRBPII) and different fluorogenic molecules, capable of supporting photobase activity. The large increase in basicity upon photoexcitation leads to protonation of the fluorophore in the excited state, dramatically red-shifting its emission, leading to an LSS protein/fluorophore complex. Essential for selective photobase activity is the intimate involvement of the target protein structure and sequence that enables Excited State Proton Transfer (ESPT). The potential power and usefulness of the strategy was demonstrated in live cell imaging of human cell lines.

PubMed: 34516091
DOI: 10.1021/jacs.1c05039

実験手法

X-RAY DIFFRACTION (1.59 Å)