7MFT

Glutamate synthase, glutamate dehydrogenase counter-enzyme complex (GudB6-GltA6-GltB6)

7MFT の概要

• エントリーDOI: 10.2210/pdb7mft/pdb
• 関連するPDBエントリー: 7MFM
• EMDBエントリー: 23817 23825
• 分子名称: Glutamate dehydrogenase, Glutamate synthase (NADPH) large chain, Glutamate synthase (NADPH) small chain, ... (7 entities in total)
• 機能のキーワード: counter-enzyme complex, glutamate synthase, glutamate dehydrogenae, cytosolic protein
• 由来する生物種: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 276358.66

構造登録者

Jayaraman, V.,Lee, D.J.,Elad, N.,Fraser, J.S.,Tawfik, D.S. (登録日: 2021-04-11, 公開日: 2022-01-05, 最終更新日: 2024-05-29)

主引用文献

Jayaraman, V.,Lee, D.J.,Elad, N.,Vimer, S.,Sharon, M.,Fraser, J.S.,Tawfik, D.S.
A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis.
Nat.Chem.Biol., 18:161-170, 2022

PubMed Abstract: Multi-enzyme assemblies composed of metabolic enzymes catalyzing sequential reactions are being increasingly studied. Here, we report the discovery of a 1.6 megadalton multi-enzyme complex from Bacillus subtilis composed of two enzymes catalyzing opposite ('counter-enzymes') rather than sequential reactions: glutamate synthase (GltAB) and glutamate dehydrogenase (GudB), which make and break glutamate, respectively. In vivo and in vitro studies show that the primary role of complex formation is to inhibit the activity of GudB. Using cryo-electron microscopy, we elucidated the structure of the complex and the molecular basis of inhibition of GudB by GltAB. The complex exhibits unusual oscillatory progress curves and is necessary for both planktonic growth, in glutamate-limiting conditions, and for biofilm growth, in glutamate-rich media. The regulation of a key metabolic enzyme by complexing with its counter enzyme may thus enable cell growth under fluctuating glutamate concentrations.

PubMed: 34931064
DOI: 10.1038/s41589-021-00919-y

実験手法

ELECTRON MICROSCOPY (3.9 Å)