7MF1

Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing antibody 47D1

7MF1 の概要

• エントリーDOI: 10.2210/pdb7mf1/pdb
• 分子名称: Spike protein S1, 47D1 Fab heavy chain, 47D1 Fab light chain, ... (6 entities in total)
• 機能のキーワード: covid-19, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71136.23

構造登録者

Yuan, M.,Zhu, X.,Wilson, I.A. (登録日: 2021-04-08, 公開日: 2021-05-12, 最終更新日: 2024-11-06)

主引用文献

Zhou, X.,Ma, F.,Xie, J.,Yuan, M.,Li, Y.,Shaabani, N.,Zhao, F.,Huang, D.,Wu, N.C.,Lee, C.D.,Liu, H.,Li, J.,Chen, Z.,Hong, Y.,Liu, W.H.,Xiao, N.,Burton, D.R.,Tu, H.,Li, H.,Chen, X.,Teijaro, J.R.,Wilson, I.A.,Xiao, C.,Huang, Z.
Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2.
Cell Rep, 35:109109-109109, 2021

PubMed Abstract: It is unclear whether individuals with enormous diversity in B cell receptor repertoires are consistently able to mount effective antibody responses against SARS-CoV-2. We analyzed antibody responses in a cohort of 55 convalescent patients and isolated 54 potent neutralizing monoclonal antibodies (mAbs). While most of the mAbs target the angiotensin-converting enzyme 2 (ACE2) binding surface on the receptor binding domain (RBD) of SARS-CoV-2 spike protein, mAb 47D1 binds only to one side of the receptor binding surface on the RBD. Neutralization by 47D1 is achieved independent of interfering RBD-ACE2 binding. A crystal structure of the mAb-RBD complex shows that the IF motif at the tip of 47D1 CDR H2 interacts with a hydrophobic pocket in the RBD. Diverse immunoglobulin gene usage and convergent epitope targeting characterize neutralizing antibody responses to SARS-CoV-2, suggesting that vaccines that effectively present the receptor binding site on the RBD will likely elicit neutralizing antibody responses in a large fraction of the population.

PubMed: 33932326
DOI: 10.1016/j.celrep.2021.109109

実験手法

X-RAY DIFFRACTION (2.092 Å)