7MEP

BG505 SOSIP.v5.2(7S) in complex with the monoclonal antibodies Rh.33172 mAb.1 and RM19R

Summary for 7MEP

• Entry DOI: 10.2210/pdb7mep/pdb
• EMDB information: 23778 23779 23780 23801
• Descriptor: RM19R mAb Light chain, RM19R mAb Heavy chain, BG505 SOSIPv5.2(7S) - gp120, ... (8 entities in total)
• Functional Keywords: immune complex, monoclonal antibodies, hiv-1, bg505 sosip, viral protein
• Biological source: Macaca mulatta; More
• Total number of polymer chains: 14
• Total formula weight: 570045.13

Authors

Antanasijevic, A.,Ward, A.B. (deposition date: 2021-04-07, release date: 2022-01-26, Last modification date: 2024-11-13)

Primary citation

Antanasijevic, A.,Bowman, C.A.,Kirchdoerfer, R.N.,Cottrell, C.A.,Ozorowski, G.,Upadhyay, A.A.,Cirelli, K.M.,Carnathan, D.G.,Enemuo, C.A.,Sewall, L.M.,Nogal, B.,Zhao, F.,Groschel, B.,Schief, W.R.,Sok, D.,Silvestri, G.,Crotty, S.,Bosinger, S.E.,Ward, A.B.
From structure to sequence: Antibody discovery using cryoEM.
Sci Adv, 8:eabk2039-eabk2039, 2022

PubMed Abstract: One of the rate-limiting steps in analyzing immune responses to vaccines or infections is the isolation and characterization of monoclonal antibodies. Here, we present a hybrid structural and bioinformatic approach to directly assign the heavy and light chains, identify complementarity-determining regions, and discover sequences from cryoEM density maps of serum-derived polyclonal antibodies bound to an antigen. When combined with next-generation sequencing of immune repertoires, we were able to specifically identify clonal family members, synthesize the monoclonal antibodies, and confirm that they interact with the antigen in a manner equivalent to the corresponding polyclonal antibodies. This structure-based approach for identification of monoclonal antibodies from polyclonal sera opens new avenues for analysis of immune responses and iterative vaccine design.

PubMed: 35044813
DOI: 10.1126/sciadv.abk2039

Experimental method

ELECTRON MICROSCOPY (3.5 Å)