7ME1

YfeA oligomer crystal 1, form 1

7ME1 の概要

• エントリーDOI: 10.2210/pdb7me1/pdb
• 分子名称: Periplasmic chelated iron-binding protein YfeA, FE (III) ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: metal coordination, metal transport
• 由来する生物種: Yersinia pestis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72460.47

構造登録者

Radka, C.D.,Aller, S.G. (登録日: 2021-04-06, 公開日: 2021-08-25, 最終更新日: 2023-10-18)

主引用文献

Radka, C.D.,Aller, S.G.
Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site.
Acta Crystallogr.,Sect.F, 77:286-293, 2021

PubMed Abstract: The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 Å resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from ∼4 to ∼16 Å away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination.

PubMed: 34473105
DOI: 10.1107/S2053230X21008086

実験手法

X-RAY DIFFRACTION (2.05 Å)