7MDT

BG505 SOSIP.v5.2 in complex with the monoclonal antibody Rh4O9.8 (as Fab fragment)

Summary for 7MDT

• Entry DOI: 10.2210/pdb7mdt/pdb
• EMDB information: 23778 23779
• Descriptor: Surface protein gp120, Transmembrane protein gp41, Rh4O9.8 monoclonal antibody Light Chain, ... (8 entities in total)
• Functional Keywords: monoclonal antibody, polyclonal antibody, immune complex, viral protein
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 8
• Total formula weight: 265419.15

Authors

Antanasijevic, A.,Ozorowski, G.,Nogal, B.,Ward, A.B. (deposition date: 2021-04-06, release date: 2022-01-26, Last modification date: 2024-11-06)

Primary citation

Antanasijevic, A.,Bowman, C.A.,Kirchdoerfer, R.N.,Cottrell, C.A.,Ozorowski, G.,Upadhyay, A.A.,Cirelli, K.M.,Carnathan, D.G.,Enemuo, C.A.,Sewall, L.M.,Nogal, B.,Zhao, F.,Groschel, B.,Schief, W.R.,Sok, D.,Silvestri, G.,Crotty, S.,Bosinger, S.E.,Ward, A.B.
From structure to sequence: Antibody discovery using cryoEM.
Sci Adv, 8:eabk2039-eabk2039, 2022

PubMed Abstract: One of the rate-limiting steps in analyzing immune responses to vaccines or infections is the isolation and characterization of monoclonal antibodies. Here, we present a hybrid structural and bioinformatic approach to directly assign the heavy and light chains, identify complementarity-determining regions, and discover sequences from cryoEM density maps of serum-derived polyclonal antibodies bound to an antigen. When combined with next-generation sequencing of immune repertoires, we were able to specifically identify clonal family members, synthesize the monoclonal antibodies, and confirm that they interact with the antigen in a manner equivalent to the corresponding polyclonal antibodies. This structure-based approach for identification of monoclonal antibodies from polyclonal sera opens new avenues for analysis of immune responses and iterative vaccine design.

PubMed: 35044813
DOI: 10.1126/sciadv.abk2039

Experimental method

ELECTRON MICROSCOPY (3.6 Å)